amittambvekar adlı kullanıcının profil görüntüsü
@amittambvekar
India bayrağı Bangalore, India
19 Mart 2012 den beri üye
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amittambvekar

Çevrimiçi Çevrimdışı
Over the 3 years, Assisting people in Molecular biology, genetics, Nutraceutical, Pharmaceutical and food businesses and companies.I am working as a professional freelancer My core competency lies in complete end-end management. I am seeking opportunities to go into partnership with excellent employers for a long term
$5 USD/hr
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Tecrübe

Research Scientist

Apr 2008

Projects undertaken: o"Cloning and expression of human Glucokinase regulatory protein gene (GCKR) in E. coli" at IBAB, o"'Studies on antioxidant and antimicrobial potential of pigments of Serratia, Pseudomonas, Micrococcus and Staphylococcus species- Skills: oAll basic molecular biology techniques (Electrophoresis, SDS PAGE, Blotting techniques, PCR, DNA- RNA-Protein isolation, Primer designing, cloning etc) oCytotoxic assays (MTT, LDH, Neutral red & Trypan blue

Eğitim

Msc

2005 - 2007 (2 years)

Nitelikler

PGDB (2009)

IBAB

BIOTECHNIQUES

Yayınlar

"Cloning and expression of human Glucokinase regulatory protein gene (GCKR) in E. coli" at IBAB

Project [login to view URL], IBAB, Dec 2009-June-2010(7-months)-Work includes ? Identification of tissue where GCKR expressed using bioinformatics tools ? Ordering cell-lines, culturing, reviving, passaging (HepG2) ? Cloning- total RNA extraction, c-DNA preparation, PCR amplification ? Designing of specific primers for PCR amplification of specific c-DNA ? Cutting plasmid with restriction enzymes, and annealing of cloned gene ? Transformation and identification of cloned cells, preservation ? expressi

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